Fig. 8. DAB-1::GFP puncta are highly dynamic. (A) Images taken from a time-lapse movie (supplementary material Movie 1) of a coelomocyte expressing DAB-1::GFP at two different time points. Overlay of the pseudocoloured images reveals that relatively few of the puncta visible at the two times overlap, indicating that most puncta initially present have disappeared by the time the second image was captured, and that most of the puncta in the second image have formed within the 76-second interval between images. (B) Images of the hypodermis of an adult hermaphrodite expressing DAB-1::GFP captured at the three different time points (see also supplementary material Movie 2). Overlay of the three pseudocoloured images demonstrates the existence of hotspots where puncta tend to form (arrows), although few of the puncta overlap. Time points are given in seconds. Bar, 2.5 µm (A); 5 µm (B).