Fig. 1. The endogenous MLN51 protein is recruited to SGs. (A) Immunofluorescence analysis of MLN51 and FMRP mobilization into SGs after arsenite treatment. HeLa cells, control (a-c, untreated) or treated with 0.5 mM arsenite for 1 hour (d-f) were either immediately fixed (a-f) or allowed to recover for 1 (g-i) or 2 (j-l) hours in normal medium without arsenite prior to fixation. Next, cells were co-labelled with anti-MLN51 (a,d,g,j, green) and anti-FMRP (b,e,h,k, red) antibodies. Nuclei were counterstained with Hoechst 33258 (blue) and corresponding merged images are shown on the right (c,f,i,l). (B) Immunofluorescence analysis of HeLa cells transiently transfected with the pEGFP-DCP1 plasmid (green). Control- and arsenite-treated cells were stained with an anti-MLN51 antibody (red), nuclei were counterstained with Hoechst 33258 (blue). Note that the endogenous MLN51 protein is not recruited into Dcp bodies.