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Figure 3


Fig. 3. Centrosome structure in anoxia. Syncytial blastoderm embryos were incubated (20 minutes) in either normoxic (+O2) or anoxic (–O2) buffer before fixation and immunolabeling. (A) Anti-Aurora A kinase (AurA), anti-{gamma}-tubulin ({gamma}-Tub) and DNA (DNA) during metaphase. (B) Anti-Centrosomin (Cnn) and DNA (DNA) during metaphase and (C) during interphase. (D) GFP-D-TACC (TACC), anti-{gamma}-tubulin ({gamma}-Tub) and DNA (DNA) during metaphase. Insets in the lower-left corners show a representative centrosome at higher magnification after labeling with anti-AurA (A) and anti-Cnn (B,C). Bars, 10 µm.