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Figure 4


Fig. 4. Motor proteins in anoxia. Syncytial blastoderm embryos were incubated in either normoxic (+O2) or anoxic (–O2) buffer for 2 or 5 minutes before fixation and immunolabeling. (A) Anti-Kinesin 8/Klp67A (KLP67A), anti-{alpha}-tubulin (Tub) and DNA stain (DNA). Klp67A decreases rapidly in the central spindle region in response to anoxia. (B) Dynein light intermediate chain-GFP (DLIC) and DNA. Dynein accumulates rapidly at metaphase plates (arrowhead) in response to anoxia. Bars, 10 µm.