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Figure 2


Fig. 2. AtKRP125c localization and expression. (A) Complementation of the rsw7 phenotype by transformation with AtKRP125c-GFP: 1-week-old wild-type (left), rsw7 (middle) and complemented rsw7 (right) seedlings grown at 19°C. Bar, 5 mm. (B-F) Confocal micrographs showing AtKRP125c-GFP in living cells of complemented rsw7 plants. AtKRP125c-GFP decorates cortical microtubules in the root (B) and hypocotyl (C), and division figures in the root, including preprophase band (D, arrow) and prophase spindle (D, arrowhead), mitotic spindle (E), and phragmoplast (F). Bars, 5 µm. (G) RT-PCR on RNA from whole 2-week-old seedlings (35 cycles). Left-hand gel: EF1-{alpha} (loading control); right-hand gel: AtKRP125c. The lane order is the same in both. Lane 1: Col genomic DNA. Lane 2: AtKRP125c-GFP genomic DNA. Lane 3: Col cDNA. Lane 4: rsw7 cDNA. Lane 5: AtKRP125c-GFP cDNA. Size standards shown in the middle.