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Figure 1


Fig. 1. ZRP-1 is localized to focal adhesions and also to cell-cell adhesions. (A) Characterization of anti-ZRP-1 polyclonal antibody. HeLa cell lysates were analyzed by western blotting with anti-ZRP-1 antibody. (B) Localization of ZRP-1 at focal adhesions. HeLa cells were stained with antibodies against ZRP-1 (left) and vinculin (middle). A merged image is shown on the right. Bars, 15 µm. (C) Localization of ZRP-1 at cell-cell contacts. Semi-confluent HeLa cells (upper panel) or saturation-density HeLa cells (lower panel) were stained with antibodies against ZRP-1 (left) and N-cadherin (middle). Merged images are shown on the right. Bars, 15 µm.