Fig. 7. Phosphorylation of focal adhesion kinase (FAK) and paxillin is suppressed in ZRP-1-depleted cells. (A) Endogenous FAK was immunoprecipitated from control or ZRP-1-depleted HeLa cell lysates. Tyr phosphorylation of FAK was detected by a phosphotyrosine antibody (4G10). (B) Endogenous FAK immunoprecipitated from control or ZRP-1-depleted HeLa cells was analyzed with the indicated phosphorylation site-specific antibodies. (C) The relative levels of total and site-specific (Tyr397, Tyr576, Tyr577, Tyr861) Tyr phosphorylation of FAK in ZRP-1-depleted cells as compared with those in control cells. (D) Endogenous paxillin was immunoprecipitated and analyzed as in (A). (E) The relative levels of total tyrosine phosphorylation of paxillin in ZRP-1-depleted cells as compared with those in control cells.