Fig. 8. Shroom2 in mouse L2071 fibroblasts stably transfected with human E-cadherin (LE cells). (A) In non-confluent LE cells that overexpress GFP-mShrm2, the protein is scattered within the cytoplasm or linked to the plasma membrane, associated with F-actin. (B-D) In confluent LE cells, GFP-mShrm2 is not recruited to the cadherin-mediated cell-cell contacts, visualized by (B) -catenin and (C,D) F-actin. Rather, the GFP-mShrm2 staining is more diffuse, and occasionally forms clusters in the (C, XZ plane) basal, or (B, XZ and YZ planes; C, YZ plane) lateral regions of LE cells. Such clusters were not observed in transfected MDCK or Caco-2 cells (see Fig. 6). XZ and YZ sections show coronal () and orthogonal () confocal reconstructions, respectively. Bars, 10 µm.