Fig. 10. Immunofluorescent staining of single-cell-derived multipotent dermal fibroblasts (MDFs). Cells were stained for vimentin (top panels) and nestin (bottom panels). Epidermal cells (EPCs) and P19 embryonal carcinoma cells (mouse neuron cell line) were used as negative and positive controls, respectively. Nuclei of all the cells are red because of the propidium iodide counterstain. Bars, 20 µm.