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Figure 5


Fig. 5. Chondrogenic induction of dermal fibroblasts. (A) After 2 weeks of chondrogenic induction in plate culture, positive staining of Safranin O was observed in the induced group (left panel) but not in the non-induced group (right panel). (B) After 3 weeks of pellet culture, immunohistochemical staining showed positive staining of collagen type II (Col II) in the induced groups (left panel) but not in the non-induced group (right panel). (C) RT-PCR shows the mRNA expression of aggrecan after 1 week of induction and collagen type II mRNA expression after 2 weeks of induction. Bars, 250 µm.