Fig. 9. Differentiation potential of lineage-committed cells. Tripotent clones were osteogenically induced for 3 weeks, followed by adipogenic induction for another 3 weeks. Parent cells show positive staining for Alizarin Red (ARS) after osteogenic induction (A), and positive staining for Oil Red O after adipogenic induction (B), whereas osteogenically induced cells fail to exhibit Oil Red O staining after switching to adipogenic medium for 3 weeks (C). Bars, 100 µm.