Fig. 7. Ran_Q69L addition cannot overcome the arrest of NPC assembly caused by inhibition of membrane fusion. Sperm chromatin was incubated with the 200,000 g supernatant of egg extract. Reactions were performed after addition of buffer, 5 µM RanQ69L, Ran_Q69L plus 100 µM -SNAP_L294A or Ran_Q69L plus 0.1% Triton X-100, as indicated. Samples were fixed and chromatin, membranes and FxFG-containing nucleoporins were visualized with DAPI, DiOC₆ or by fluorescently labeled mAb414 antibody, respectively. Note the increased recruitment of membrane and FxFG-nucleoporin in the presence of Ran_Q69L, but not with Ran_Q69L in combination with detergent or excess -SNAP.