Fig. 9. Micrococcal nuclease assay. (A) Agarose gel electrophoresis of genomic DNA from MSCs transduced with Ad-CMV and Ad-CMV-Brg1. DNA was digested with micrococcal nuclease for various times, as indicated. The picture shows a high molecular weight DNA band that is more resistant to nuclease digestion in Ad-Brg1 samples than in Ad-CMV samples (see arrow). (B) DNA resolved on agarose gel following nuclease digestion as in A. MSCs were transduced with Brg1 and with viruses carrying E1A, E1A-YH47 and E1A-RG2 isoforms. These inhibit both p53 and Rb (E1A) or p53 (E1A-YH47) or Rb (E1A-RG2) alone. The picture shows high molecular weight DNA bands and faint smears of digested DNA molecules (see arrows).