Fig. 1. The amphipathic tail-anchoring peptide (ATAP) is conserved in Bfl1 and HCCS1. (A) Schematic genomic structures of BCL2A1 (BFL-1) and HCCS1 genes on human chromosome 15q24.3 and 15q25.1, respectively. Black bars indicate exons and red bars indicate conserved exon-3 of BCL2A1 and HCCS1 genes. (B) Alignment of exon-3 sequences from BCL2A1 and HCCS1 genes. Identical sequences are shaded gray and single base gap is shaded black. Stop codons are yellow. (C) Primary sequence comparison of the TA region of Bfl1 and HCCS1. Horizontal red bar indicates the ATAP sequence of Bfl1 and HCCS1. Green lines indicate predicted -helical regions. Mitochondrial targeting signal (MTS) of HCCS1 is indicated by a blue line. (D) Helix-wheel diagrams of ATAP sequences of Bfl1 and HCCS1. (E) Amino acid sequence alignment of TA regions from human anti-apoptotic Bcl2 family proteins and A1, a mouse homologue of human Bfl1, and ATAP sequences.