Fig. 2. Transcript characterization. (A) Northern blot showing the two sizes of noa mRNA of female (F) and male (M) OreR flies after hybridization with a noa cDNA next to a northern blot that was hybridized with the PCR fragment indicated in Fig. 1B. Transcript sizes are given in kb on the left. (B) RNaseH analysis. OreR mRNA was hybridized with oligo(dT), RNaseH treated to varying degrees (+, ++) and analyzed with a noa cDNA probe in parallel with untreated samples (–). The size of the different cleavage products is indicated in kb at the side of the blots.