Fig. 5. Expression analysis. (A) Expression pattern in the germarium at the beginning of oogenesis is shown via the enhancer trap function. Germ cells show -galactosidase activity in all regions of the germarium (1-3) and in all follicle stages (e.g. S2). (B-H) In situ hybridizations to noa sequences are shown for a stage 10 follicle (B), an embryo at the syncytial blastoderm stage expressing maternal noa RNA (C), an embryo at the cellular blastoderm stage (D,E) with apical localization of the transcripts (black arrow in E) and pole cells without noa transcripts (white arrow, E), and an embryo around stage 8-9 with expression in the central nervous system (F) and in the PNS (G). Two imaginal discs (wing and leg) also contain noa transcripts (H). (I) Expression in a third instar larval brain is detected by X-Gal staining after expression of a noa-lacZ reporter gene.