Fig. 7. Noa expression in the adult testis. (A) A group of cells in the testis tip of the enhancer trap line exhibits -galactosidase activity. (B) Confocal microscopy after staining with anti-Fasciclin III antibody (to locate the hub cells, red) and anti--galactosidase antibody (green) proves close proximity of the two cell types. (C) Both head (arrowhead) and tail (arrow) cyst cell express -galactosidase in an isolated cyst with elongating spermatids. (D) Nuclei are visualized using Hoechst 33258 staining. (E) In situ hybridizations on spermatid bundles are shown. The noa transcripts are found along the spermatid bundles, i.e. in the cyst cells (top). For comparison, the germ cell-specific transcripts of Mst87F are detected within the spermatid bundles (bottom). (F) NOA-lacZ fusion proteins are also detected around the spermatid bundles and not within the bundles.