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Figure 2


Fig. 2. Movement of human PTECs transfected with GFP-VASP and GFP-S239AVASP. (a) Sequence of frames taken from Movie 1 (see supplementary material), showing one cell imaged at the indicated intervals (in minutes). The final frame (t=65 minutes) shows the outline of the original cell at its starting position at t=0 minutes. The white line indicates the axis used to generate the kymograph in panel c. (b) Still frames from another cell transfected with WT GFP-VASP and imaged at the indicated times (in minutes) after the start of the experiment. The white line indicates the axis used to generate the kymograph in panel d. (c) Kymograph of the cell shown in panels a, taken along the axis indicated by the white line. (d) Kymograph of the cell shown in panels b, taken along the axis indicated by the white line. (e) Sequence of frames from Movie 2 (see supplementary material), showing one cell imaged at the indicated intervals (in minutes). The final frame (t=64.5 minutes) shown the outline of the original cell at its starting position at t=0 minutes. The white line indicates the axis used to generate the kymograph in panel g. (f) Still frames from another cell transfected with S239A GFP-VASP and imaged at the indicated times (in minutes) after the start of the experiment. The white line indicates the axis used to generate the kymograph in panel h. (g) Kymograph of the cell in shown panels e, taken along the axis indicated by the white line. (h) Kymograph of the cell shown in panels f, taken along the axis indicated by the white line. Bars, 10 µm. (i) Box and whisker plot of cell migration and lamellipodial projection speeds for human PTECs transfected with WT GFP-VASP and S239A GFP-VASP (WT and S239A, respectively). Horizontal line is the median value, the box encloses the 25th and 75th percentiles. The bars indicate the range of the values. The differences in cell migration and lamellipodial projection speeds between WT and S239A were not significant (n.s.) as determined by Mann-Whitney test; n=7-12 independent observations.