Fig. 6. Effects of the phosphomimetic S239D mutation on VASP distribution. (a) Distribution of WT GFP-VASP and S239D GFP-VASP fusions (green) in human PTECs with corresponding phase-contrast views. Edges of lamellipodia are shown by arrowheads. Bar is 10 µm. (b) Quantification of VASP in lamellipodia. Percentage of lamellipodia containing VASP measured as described in the Materials and Methods for WT GFP-VASP (WT; n=30) and S239D GFP-VASP (SDT; n=61). ^*P<0.0001 (Fisher's exact test), significant difference from WT GFP-VASP.