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Figure 3


Fig. 3. UNC-87 and tropomyosin inhibit actin filament severing by UNC-60B. Alexa Fluor 488-biotin-labeled rabbit actin filaments in perfusion chambers were treated in two sequential perfusion steps with a buffer containing proteins as indicated on the figure. Arrows in j indicate severed filaments. Micrographs of the same fields were taken after both perfusion steps. Bar, 20 µm.