Fig. 3. Post-transcriptional repression of HSP60 and HSD70 by miR-1 and caspase-9 (Casp9) by miR-133. (A-C) Western blot analysis of HSP60, HSP70 and Casp9 (total) under various conditions with protein samples from H9c2 cells. The bar charts in the lower panels represent the densitometric measurements of western blots of HSP60, HSP70 and Casp9 expression. (D) Effects of miR-133 on Casp9 activity. (E) Effects of AMO-1 and AMO-133, respectively, on protein levels of HSP60, HSP70 and Casp9 in H9c2 cells. (F) Effects of miR-1 and miR-133 on mRNA levels of HSP60, HSP70 and Casp9 in H9c2, as determined by real-time RT-PCR. Data presented as means ± s.e.m. (n=8, 6, 6, 5, 6, and 6, for A-F, respectively). ^*P<0.05 vs Ctl; ⁺P<0.05 vs WT miR-1 or WT miR-133.