Fig. 6. Comparison of miR-1 and miR-133 expression levels under various conditions, measured by real-time RT-PCR. (A) miR-1 and miR-133 levels without and with transfection of exogenous miR-1 and miR-133. The data are averaged from three batches of H9c2 cells. ^*P<0.05 vs non-transfected cells. (B) Enhanced expression of miR-1 and miR-133 induced by H₂O₂ (150 µM) in H9c2 cells (n=5 batches of cells for each group). ^*P<0.05 vs Ctl; ⁺P<0.05 vs miR-1 or miR-133. (C) Comparison of miR-1 and miR-133 expression levels in various cell lines indicated. H9c2, rat ventricular cell line (n=6 batches of cells); A549, human lung cancer cell line (n=7 batches of cells); HEK293, human embryonic kidney cell line (n=7 batches of cells). MiR-1 and miR-133 levels are expressed as relative levels by normalizing to the values obtained from H9c2 cells. ^*P<0.05 vs H9c2.