Fig. 2. CD81 partially colocalizes with v5 integrin receptors at the apical surface of RPE cells. Live RPE-J cells were labeled with either CD81 antibody Eat2 (left column) or v5 receptor antibody P1F6 (middle column), or both antibodies (right column). All samples received both secondary antibodies and were examined at both corresponding wavelengths to ensure labeling specificity of secondary antibodies. CD81 signals are shown in green in the middle row, v5 signals are shown in red in the top row. The bottom row shows the merged signals of upper and middle rows. Panels show representative single x-y plane confocal microscopy scans of the RPE-J apical surface. Areas with focus on the apical surface of cells showed considerable colocalization of CD81 and v5, appearing in yellow in the merged images. Selected areas are enlarged and shown in the fourth column to better illustrate co-localization. Bars, 10 µm.