Fig. 8. FRAP analyses of GFP-ninein at the centrosome in PtK2 cells. (A) Images from a time-lapse recording showing recovery of GFP-ninein at the centrosome following photobleaching (arrows). (B) Images from a time-lapse recording of a cell pre-treated with nocodazole showing no significant recovery at the centrosome (arrows) following photobleaching. (C) Graphs depicting the fluorescence intensity during recovery following photobleaching of the control cell in A (red line) and the nocodazole-treated cell in B (blue line). The fluorescence intensity at the centrosome following photobleaching was normalised to that prior to photobleaching. (D) Histogram comparing percentage centrosomal GFP-ninein fluorescence recovery 4 minutes after photobleaching in control (red; 98.48±1.52%, n=5) and nocodazole (blue; 23.83±3.68%, n=6) treated cells.