Fig. 8. Overexpression of an APC-independent form of -catenin leads to functional bypass of mDiaN3 inhibition. (A) Myoblasts were co-transfected with a control plasmid (GFP), N3+GFP or N3+ -catenin S37A and TCF activity determined (mean ± s.e.m., n=5, P<0.0046). (B) MyoD expression in cells transfected as in A. Note that N3+ -catenin S37A transfected cells retain the elongated morphology typical of N3 transfectants but are MyoD⁺. (C) Quantification of MyoD expression in myoblasts transfected with either N3+ -catenin S37A or N3HindIII+ -catenin S37A. The degradation-resistant -catenin S37A mutant partially reverses the inhibition of MyoD expression mediated by both mDia derivatives. (mean ± s.e.m., ^**P<0.0002, n=6; ^*P<0.046, n=3).