Fig. 1. Disruption of epithelial structure and adherens junctions in onion rings mutant embryos. (A-C) Neurotactin localizes to basolateral cell surfaces in wild-type (A) and onr mutant embryos (B,C). At stage 6, onr mutants establish columnar epithelial morphology normally (B), whereas stage 10 onr mutants display a severe epithelial disruption (C) compared to the wild type (A). (D-I) At stage 10, Armadillo (green D-F) and DE-cadherin (green G-I) localize apically in the wild type (D,G) and in an onr mutant carrying the P[Exo84] transgene (I). onr mutants accumulate Armadillo (E,F) and DE-cadherin (H) at various positions along the apical-basal axis. Neurotactin (red D-F) and filamentous actin (F-actin red G-I) are enriched at basolateral surfaces in wild-type and onr mutant embryos. (F) A 5 µm projection of multiple optical sections stained for Armadillo (green), superimposed on a single 1 µm slice of the cell outline marker Neurotactin (red). Anterior, left; ventral, up. Bar, 20 µm.