Fig. 3. Mislocalization of apical proteins in Exo84 mutant embryos. (A-J) Bazooka (green A,C-E,G-J), Crumbs (green B,F), aPKC (blue C,G) and dPATJ (red D,H) localize to the apical margin of lateral cell surfaces in the wild type (A-D,I), but form ectopic aggregates in Exo84 mutants (E-H,J). Dlg (red I,J) is excluded from the apical surface in the wild type (I). (J) Exo84 mutants display disrupted Bazooka localization in all cells pictured, whereas apical Dlg exclusion is often maintained. Neurotactin (red A,C,E,G) delineates cell outlines and DAPI (purple B,F) labels nuclei. (K,L) Armadillo (green K,L) and Bazooka (red K,L) colocalize in wild-type adherens junctions (K) and in basolateral aggregates in Exo84 mutants (L). (M-P) In the wild type (M,N), Armadillo (red) and Bazooka (blue) localize to adherens junctions (M) and are not detected basally (N, 4 µm below M). In Exo84 (O) and crumbs mutants (P), Armadillo (red) and Bazooka (blue) are mislocalized along the apical-basal axis. All embryos are stage 10. Anterior, left; ventral, down. Bar, 20 µm (A); 5 µm (K).