Fig. 6. The recycling endosome compartment is disrupted in Exo84 mutants. (A) Schematic of results depicting the distribution of Golgi (Lva), early endosome (Rab5), late endosome (Hrs) and recycling endosome (Rab11) compartments in cross section in wild-type and Exo84 mutant embryos. (B-J) Localization of Lva (red B,C), Rab11 (green D-F), Rab5-GFP (green G,H), Hrs (green I,J), Armadillo (green B,C) and Bazooka (blue B,C,G-J, red D-F) in stage 10 wild-type (B,D,G,I), Exo84 (C,E,H,J) and crumbs mutant (F) embryos. (B,C) The Golgi compartment (Lva, red) appears normal in size and distribution in the wild type (B) and Exo84 mutant (C). (D-F) Recycling endosomes (Rab11, green) are diffusely distributed in the apical cytoplasm in wild type (D), but form large aggregates in Exo84 mutants (E). Despite disruption of epithelial polarity and Bazooka localization (red) in crumbs mutants (F), recycling endosomes are comparable in appearance to the wild type (D). (G-J) Early endosomes (Rab5-GFP, green G,H) and late endosomes (Hrs, green I,J) are distinct compartments from recycling endosomes (Rab11, red) in the wild type (G,I) and Exo84 mutant (H,J). Bazooka aggregates (blue) colocalize with recycling endosome aggregates. Anterior, left; ventral, down. Bar, 10 µm (B); 5 µm (G,I).