Fig. 6. Depletion of Mlph together with interacting proteins Rab27a and MyoVa, but not EB1, causes clustering of wild-type melanosomes. Wild-type melan-ink melanocytes were transfected with the siRNA oligonucleotides that specifically prevent the synthesis of the indicate proteins. Transfected cells were fixed 72 hours later and stained with the indicated specific antibodies to allow correlation of depletion of protein to melanosome distribution. Panels A, C, E, G, I, K and M are fluorescence images showing the expression and distribution of the indicated protein in transfected cells and panels B, D, F, H, J, L and N are transmitted light images showing the distribution of pigment granules in transfected cells. Bar, 20 µm.