Fig. 7. siRNA-mediated depletion of individual Rab27a-Mlph-MyoVa complex components affects the overall stability of the complex. Pools of wild-type melan-ink melanocytes were transfected with the indicated siRNA oligonucleotide, harvested 72 hours later and lysates subjected to immunoblotting using the indicated antibodies. Transfections were carried out in triplicate and the migration of molecular-weight standards is indicated on the left of each blot.