Fig. 6. Chromosome cores disassemble in the absence of ORD activity. SMC1 (top two rows of panels) and SMC3 (bottom two rows panels) immunostaining are shown for single ord⁺ and ord^null nuclei within indicated regions of the germarium. In early region 2A, continuous SMC1 and SMC3 threads are visible in both mutant and wild type. As cysts mature, thread-like SMC staining becomes severely disrupted in the mutant. Only dim fragments or puncta are observed in region 2B ord^null oocytes, and by region 3 mutant nuclei often contain no visible signal. Note that ord^null nuclei lack bright SMC1 and SMC3 foci. Images are projections of deconvolved Z-series of whole-mount preparations. For each antibody, wild-type and mutant images for each stage were captured and processed identically. Bar, 1 µm.