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Figure 3


Fig. 3. RASSF1A binds Ras. (A) HEK-293-T cells were transfected with FLAG-tagged RASSF1A and HA-tagged forms of K-Ras12v. The cells were lysed and immunoprecipitated (IP) before being immunoblotted (IB) with HA and FLAG. Upper panel shows immunoprecipitation, lower panel shows protein levels in the cell lysate. Wild-type K-Ras, a Y40C effector mutant of K-Ras12v and a farnesylation-defective mutant of K-Ras12v (K-RasCX) were defective for binding RASSF1A.