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Figure 2


Fig. 2. The internalized cell-surface tTG is transported through endosomal compartments to lysosomes. Antibody-uptake experiments were performed as described above in Fig. 1A. Double-immunofluorescence staining of WI-38 fibroblasts for internalized tTG–4G3-Fab complexes (central panels) and endocytic markers (left panels), including EEA1 (early endosomes, 10 minutes of endocytosis), Rab7 (late endosomes, 30 minutes of endocytosis), Arf1 (ECV/Golgi, 30 minutes of endocytosis) or Lamp-1 (lysosomes, 120 minutes of endocytosis). Merged images are shown in the right panels. Arrows indicate colocalization of the internalized tTG–4G3-Fab complexes with various organelle markers.