Fig. 3. Colocalization of punctate VSVG-ts045-GFP staining with ERGIC p53. (A-F) Huh7 cells were transfected with VSVG-ts045-GFP-expressing plasmid and infected with poliovirus as in Fig. 2. After 3 hours of infection at 40°C, the cells were shifted to 32°C and incubation continued for either 20 minutes (A-C) or 60 minutes (D-F). After fixation, cells were labeled with an anti-ERGIC p53 antibody and visualized by confocal microscopy for indirect immunofluorescence of ERGICp53 (A,D) and GFP fluorescence (B,E). (C,F) Merged images.