Fig. 7. Loss of both Apc6/Cdc16 and Apc8/Cdc23 functions induces apoptosis. Orcein stained preparations from brains of Apc6/Cdc16 (A) and Apc8/Cdc23 (B) RNAi L3 larvae show small, rounded cells usually in pairs with intense but uneven nucleolar staining resembling apoptotic cells. Acridine Orange staining further indicates the high incidence of dying cells in brains of RNAi larvae (D, only an Apc8/Cdc23 RNAi sample is shown) relative to wild-type larvae (C). (E) Bar graph showing the apoptotic index in neuroblast preparations of the respective genotypes. The apoptotic index is given as the mean number of apoptotic cells in a microscope field.