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Figure 4


Fig. 4. Chromatin remodelling can be induced in MRC5 fibroblasts by IFN{gamma} and sodium butyrate (SB). (A) FISH image showing HLA-DRA (red) extending from the painted CT6 (green) in MRC5 cells after treatment with SB for 1 hour. (B) Comparison of external chromatin loops for the MHC and the 6p24 region in untreated control cells, in cells treated with IFN{gamma} for 24 hours and in cells treated with SB for 1 hour (n>=100). A significant increase in the percentage of external chromatin loops was observed for all three MHC regions in IFN{gamma} and SB-treated fibroblasts compared with untreated cells. (C) External chromatin loops carrying HLA-DRA induced by SB treatment over a 1-hour time course. (D) Comparison of external chromatin loops induced by IFN{gamma} over a 24-hour time course in control cells (without IFN{gamma}) and cells treated with SB for the last 1 hour of the IFN{gamma} treatment. The mean percentage of signals on external chromatin loops is represented on the y-axis, error bars show the standard deviation. The value at the 0-minute (0') time point for MRC5+SB+IFN{gamma} is high because the cells have been exposed to SB for 1 hour.