Fig. 4. Modulations of membrane potential (V_m), K⁺ outward rectifying currents (KORCs) and anion currents of A. thaliana cells in response to 200 nM HrpW_ea or HrpN_ea. (A) Free-running V_m observed in response to HrpW_ea, HrpN_ea or the desalting buffer. (B) Mean values of the V_m variations. (C) Effect of 200 nM HrpW_ea on KORCs recorded at +80 mV (leak substracted). The activated currents were measured when the maximum effect of HrpW_ea was reached. V_h, holding potential; V_m, membrane potential. (D) Current-voltage relationships. The steady-state current amplitudes were measured at membrane potentials ranging from –200 to +80 mV. (E) Mean amplitudes of steady state KORCs (at 80 mV) in response to 200 nM HrpW_ea, HrpN_ea alone, HrpN_ea and 10 mM TEA or the corresponding desalting buffer volume. Values are given as a percentage with respect to current value before treatment. (F) Effect of 200 nM HrpW_ea on anion current recorded at –200 mV. Currents were measured when the maximum effect of HrpW_ea was reached. (G) Corresponding current-voltage curves. (H) Mean amplitudes of anion currents in response to 200 nM HrpW_ea alone, HrpW_ea and 10 µM Glibenclamide or 40 µM 9AC, 200 nM HrpN_ea or the corresponding desalting buffer volume. Values are given as a percentage with respect to current value before treatment. All values are mean of at least five independent experiments. Error bars represent s.e.