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Figure 5


Fig. 5. NO scavenged by OxyHb is associated with a delay in cell death during chronological life span and to decreased levels of superoxide anion. (A) Growth curve of wild-type cells after addition of indicated concentrations of OxyHb. (B) Survival determined by clonogenicity during chronological aging of wild-type cells with or without addition of OxyHb, at the indicated concentrations on day 0. (C) Quantification (fluorescence) of ROS accumulation using dihydroethidium (DHE) staining during chronological aging of wild-type cells with or without OxyHb treatment.