Fig. 2. Septate junction components and lateral markers in the AS and epidermis during DC. Stage-13 (A) and stage-15 (B) embryos stained for the basolateral marker Dlg (Aii, Bii, red) and NrxIV (Aiii, Biii, green) showing that AS cells do not show SJ proteins at the membrane. Dlg (arrowhead in Aii), but not NrxIV (arrowhead in Aiii, Biii), is present at the LE. (Ci,Di) Thick sections of a stage-13 (Ci) and stage-14 (Di) embryo stained for the basolateral marker Scrib (Cii, Dii, red), E-cadherin (ECad; Ciii, Diii, green) and FasIII or Dlg (Cvi, Dvi, white), confirming that basolateral markers (Cii, Dii, Dvi, arrowheads) are localized at the LE but that SJ-associated components (Cvi, arrowhead) are absent from the LE. (Cv, Dv) Schematic representation of the image in Ci. pAS, marginal AS cell; m, mesoderm; e, endoderm. (Ei) Stage-13 embryo expressing NrxIV with the daGal4 driver, stained for FasIII (Eii, red) and Nrx (Eiii, green). Notice the localization of Nrx at the membrane of AS cells (arrow) and at the LE (arrowheads).