Fig. 3. Roles of AJ during DC. (Ai) shg^R64 embryos stained for actin (Aii, phalloidin-TxR), E-cadherin (ECad; Aiii, green), Armadillo (Arm; Aiv, white) and -catenin (-cat; Av, green). Aii-Aiv are single-channel images of the boxed area in Ai; Av is a different embryo. Note the disruption of the actin cable (Aii, arrowhead) and the reduction of ECad and Arm specially at the LE (Aiii, Aiv, arrowheads). (D) shg^R64 embryo overexpressing ActinGFP in the engrailed domain. Note the presence of mismatches (arrowhead). The inset shows the filopodia formed by DME cells in these mutant embryos (arrowhead). (Bi) shg^g317 embryos stained as in Ai, showing the dislodgement of the AS from the epidermis. The actin cable (Bii, arrowhead) is absent and the levels of membrane ECad (Biii, the arrowhead points at the LE), Arm (Biv), and -catenin (Bv) are very much reduced in the epidermis. (Bii, Biii) Single-channel images of the boxed area in Bi; (Biv and Bv) single-channel images from a different embryo. (E) shg^g317 embryo over-expressing ActinGFP in the engrailed domain showing the dorsal hole characteristic of these embryos. The inset shows the very small filopodia formed in these mutants (arrowhead). (Ci) arm^YD35 embryos stained as in Ai, showing the detachment of the AS from the epidermis. In contrast to shg mutants, these embryos form an actin cable (Cii, arrowhead) and the levels of ECad (Ciii) remain high in the AS and epidermis. Arm levels are lower in both tissues (Civ) and -catenin is mainly cytoplasmic (Cv). F, arm^YD35 embryo overexpressing ActinGFP in the engrailed domain showing a dorsal hole. The inset shows that normal filopodia are formed in these mutant embryos (arrowhead).