Fig. 5. Interactions between E-cadherin (ECad) and Arm are required during DC. (A) Schematic representation of a fusion protein, in whih -catenin is fused to a form of ECad that lacks the Armadillo (Arm)-binding domain (ECadβ–-catenin) and thus is not able to recruit Arm (Cii). (B) Cuticle of a shg^g317 embryo expressing ECadβ–-catenin in the engrailed domain showing the lack of rescue of the dorsal open phenotype. (Ci) Embryo of the same genotype as in B, stained for actin (phalloidin-TxR), ECad (green) and Arm (blue, shown as a separate channel in Cii. Notice the lack of recruitment of Arm in the engrailed stripes) showing that the expression of the ECadβ–-catenin is not able to rescue the adhesion between the AS and the epidermis. (D) Schematic representation of the ECadFL–-catenin chimera in an Arm-mutant background. (E) Cuticle of an arm^YD35 embryo showing the dorsal open phenotype. (F) Cuticle of an arm^YD35 embryo expressing ECadFL–-catenin in the engrailed domain. No rescue of the dorsal hole is observed. (G) Embryo of the same genotype as in E, stained for actin (phalloidin-Txr) and ECad (blue). No rescue of the adhesion between the AS and the epidermis is observed (arrowhead).