Fig. 5. Overexpressed full-length CAP350 GFP localises to the centrosome and to a subset of pericentrosomal microtubules. (A-C) Full-length CAP350 GFP overexpressed in cells and stained for -tubulin. At low expression levels, GFP decorates the centrosome (A). When the expression level increases, a subset of microtubules close to the centrosome is covered with CAP350 (B) and extended further in the cell (C). (D) 2.5x magnification of the boxed area in C. Note that CAP350 GFP is not continuous on microtubules, and that where it is present the anti-tubulin antibody has no access to MTs (arrows). A microtubule well stained with the anti-tubulin is only partly decorated with the fusion protein (arrowhead). Bar, 20 µm.