Fig. 8. Effect of CAP350 depletion or overexpression on the Golgi complex. Cells transfected with the GL2 (A) or CAP350 (B) duplexes for 48 hours were fixed with methanol and stained for CAP350 and CTR433 a median Golgi marker. Cells transfected with the GL2 duplex present a classical Golgi complex. After depletion of CAP350 with the siRNA CAP350, the Golgi complex appeared scattered. (C-D) Representative cell expressing CAP350-GFP and stained for CTR433 (C) or A24, an antibody directed against AKAP450 (D). Note that the cell overexpressing CAP350-GFP presented a scattered Golgi complex in contrast to the untransfected cells. Interestingly, the Golgi-like network marker AKAP450 partially redistributed and colocalised with CAP350. Bars, 20 µm