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Figure 2


Fig. 2. Strong correlation between electrotactic migration and EGFR (ErbB1) expression levels. (A) A small EF of 0.5 V/cm was applied to cells for 2 hours. Non-metastatic rat mammary adenocarcinoma MTC clone (13762NF) showed weak electrotaxis, whereas the high metastatic clone, MTLn3 showed robust electrotaxis. There were significant quantitative differences in migration speeds and in cell directedness. *P<0.001 compared with MTC cells. Data are mean ± s.e.m. of three independent experiments. (B) Significantly varied expression levels of ErbB1 in five breast cancer cell lines were detected by western blot. {alpha}-tubulin is loading control. (C) ErbB1 expression level (upper histogram) correlates strongly with the directionality of EF-directed migration (lower histogram). Cells were starved for 3 hours, and then a small EF was applied for 2 hours (0.5 V/cm for rat cell lines, 1.0 V/cm for human cell lines).