Fig. 3. ErbB1 expression enhances electrotactic responses. MTC and MTC-B1 cell lines were starved for 3 hours, and then 0.5 V/cm EF was applied for 2 hours. (A) Non-metastatic MTC cell line did not show electrotaxis at 0.5 V/cm (upper panel), after ErbB1 transfection (MTC-B1), cells showed significant strong electrotaxis (lower panel). Red lines and yellow arrowheads represent trajectories and direction of cell movement. (B) Bar graphs showing that ErbB1-transfected cells (MTC-B1) move faster and with greater anodal directedness than MTC cells. *P<0.01 compared with MTC parental cells. Data are mean ± s.e.m. from three independent experiments.