Fig. 5. RGS19 overexpression attenuates -catenin stabilization that results from Wnt3a stimulation. (A) F9 cells transiently transfected with Fz1 and either empty vector or RGS19 were treated with Wnt3a for the indicated lengths of time. Lysates were collected and treated with Con A to separate cytosolic from membrane-associated -catenin. Cleared samples were subjected to SDS-PAGE on an 11% acrylamide gel, transferred to nitrocellulose and probed with an antibody against -catenin. The increase in cytosolic -catenin that is seen in response to Wnt3a is attenuated by overexpression of RGS19 (+ RGS19). The results displayed are from a single experiment representative of more than three independent tests. IB, immunoblotting. (B) Bands from multiple experiments were quantified by densitometry. The data shown are mean values (±s.e.) of at least three separate experiments. *P<0.05 for the difference between wild-type cells and those expressing RGS19 for the indicated time points.