Fig. 5. Effect of HAT and HDAC inhibitors on HP1 localization. (A) Distribution of HP1-GFP and HP1-GFP in C127 cells treated with buffer (NT), curcumin (Cu), Trichostatin A (TSA) and sodium butyrate (NaBu). Note that HDAC inhibitors cause randomization of HP1 proteins in a subset (30%) of cells. The same results are obtained with HeLa cells (not shown). (B) FRAP data from cells treated with NaBu and exhibiting the diffuse (D) phenotype. Fluorescence recovery half-times (t_1/2) are in seconds; im. fr. is the immobile fraction. Note that the immobile fraction disappears and the fluorescence recovery half-times become shorter in comparison to those of non-treated cells (see Fig. 4F). Bar, 5 µm.