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Figure 2


Fig. 2. Localisation of GFP-RCC1{alpha} and GFP-RCC1{gamma} in U2OS cells. (A) Representative deconvolved images of different cell cycle stages of live cells transiently co-transfected with GFP-RCC1{alpha} or GFP-RCC1{gamma} and RFP-histone H2B. Bars, 12 µm. (B) Proportion of live cells transiently transfected with each GFP-RCC1 isoform with each pattern of localisation (left), shown as mean percentages from three experiments ± s.d., with over 1200 cells for each parameter. Examples are shown (right). Bar, 20 µm.