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Figure 3


Fig. 3. RCC1{gamma} has a more stable interaction with chromatin in U2OS cells than RCC1{alpha}. (A) FRAP of live interphase cells expressing GFP-RCC1 isoforms. Example images from one photobleach are shown, with the bleach spot indicated by an arrow. Mean half-times of recovery for each isoform in seconds are shown, ± s.d., calculated from 90 cells for GFP-RCC1{alpha} and 63 cells for GFP-RCC1{gamma} over three separate experiments. (B) Subcellular fractionation. Soluble nuclear markers are Ran, Crm1 and importin beta; cytoplasmic marker is GAPDH; and insoluble nuclear marker is lamin B. (C) Localisation of GFP-GST-NTR{alpha} (GG-NTR{alpha}), GFP-GST-NTR{gamma} (GG-NTR{gamma}) and GFP-GST constructs in fixed cells. Values shown are from >22 mitotic cells for each construct counted over four experiments. Bar, 16.3 µm.