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Figure 5


Fig. 5. Phosphorylation of RCC1 isoforms. (A) Subcellular fractionation of mitotic (M) and asynchronous (A) HeLa cells. Soluble nuclear markers are Ran, Crm1 and importin beta; cytoplasmic marker is GAPDH; insoluble nuclear marker is lamin B; and mitotic chromatin marker is phospho-S10 histone H3. (B) Alignment of the total RCC1 and pS11 RCC1 from SDS-PAGE and immunoblotting of fractionated chromatin pellet run in triplicate. (C) Phosphorylation of untagged RCC1{alpha} and RCC1{gamma} by p13Suc1 precipitates of CDKs from mitotic HeLa extract, or GS4B precipitates as a control. Molecular mass (in kDa) is indicated on the left.